powerful 1D gel analysis software which can import TIF images from different units and completely evaluate the gel data in a qualitative and quantitative way
running under OS: Win XP, 7
Software functions:
import of uncompressed greyscale TIF files in 8 -16 bits
intensity calibration by means of greyscale wedge
analysis of 3 different types of gels
fully automated lane detection with adjustment of the lane width and to the gel distortion
manual detection of the lanes and correction of the lane geometry
background subtraction according to different methods
automated and manual band detection
function "Gaussian approach" for the calculation of the volume distribution of non-specifically separated bands
molecular weight determination with one or more standards
Rf calibration (important for comparisons between gels)
normalisation according to 3 different methods
quantification (assignment of real quantities to arbitrarily many bands, to the bands of one lane or the applied quantity per lane)
band matching (assignment of comparable bands, e.g. RFLP, dilution series) on the basis of the band position, the Rf and MW values or manual settings
creation of dendrograms of the lanes of the experiment using the algorithms "Neighbour Joining" and "UPGMA"
markings
selectable print functions and GLP-conform report printouts (analysis proof)
export of the image and table data via clipboard, export of the protocols as RTF
file navigation and experiment navigation system
storage of specific evaluations as analysis protocols (template) for an automated evaluation of gel series